Sahiwal seminal plasma extracellular vesicles (LF1) - SRA

SRX25729296: Sahiwal seminal plasma extracellular vesicles (LF1)
1 ILLUMINA (Illumina NovaSeq 6000) run: 25.2M spots, 7.6G bases, 2.3Gb downloads

Design: RNA sequencing libraries were prepared with Illumina-compatible NEBNext Ultra II Directional RNA Library Prep Kit. 5l of the total RNA was used for fragmentation and priming. Fragmented and primed RNA was further subjected to first strand synthesis followed by second strand synthesis. The double stranded cDNA was purified using NEBNext purification beads. Purified cDNA was end-repaired, adenylated and ligated to Illumina multiplex barcode adapters as per NEBNext Ultra II Directional RNA Library Prep protocol followed by second strand excision using USER enzyme at 37 C for 15mins. Adapter ligated cDNA was purified using NEBNext purification beads and was subjected to12 cycles for Indexing- (98C for 30 sec, cycling (98C for 10sec, 65C for 75sec) and 65C for 5min) to enrich the adapter-ligated fragments. Final PCR product (sequencing library) was purified with JetSeq Beads, followed by library quality control check.

Submitted by: Animal Biotechnology Division, National Dairy Research Institute

Study: Small RNA sequencing

PRJNA1147473 • SRP526766 • All experiments • All runs

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Sahiwal HF1, Sahiwal HF2, Sahiwal HF3, Sahiwal LF1, Sahiwal LF2, Sahiwal LF3

Sample: Sahiwal seminal plasma extracellular vesicles (LF1)

SAMN43172760 • SRS22369395 • All experiments • All runs

Organism: Bos indicus

Library:

Name: SO_10539_REPL_SWLF1_PLAN-B

Instrument: Illumina NovaSeq 6000

Strategy: OTHER